About array CGH
Array Comparative Genomic Hybridisation (array CGH) is an emerging technology that is used to analyse DNA copy number changes using DNA microarrays to determine if there is a deletion or amplification in the genomic DNA1. This technique involves comparing the copy number of an unknown DNA to a reference (or known) DNA. The DNA sample to be investigated is labelled with one fluorescent dye. The reference sample is labelled with a second fluorescent dye. The two samples are then co-hybridised to a microarray. Any change in the copy number at a particular position in the unknown sample compared to the reference will result in a change in the dye 1: dye 2 ratio for the complementary probe.1.
The DNA can be bacterial where different strains of bacterial genomic DNA can be compared for amplifications or deletions. Mammalian DNA can also be analysed by array CGH to determine the location of chromosomal genomic DNA aberrations.
1reviewed Mantripragada KK., Buckley PG., Diaz de Stahl T and Dumanski JP. (2004) Trends Genet. 20 p87-94
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