OGT customised ChIP microarrays

Our technology and scientific knowledge enables us to produce customised chromatin immunoprecipitation (ChIP) arrays to identify precisely where proteins of interest such as transcription factors, histones and polymerases bind to their targets in living cells.

Summary of the OGT ChIP service

1. Design and optimisation of the array probes to any genome
2. Fabrication of the customised oligonucleotide ChIP arrays using ink jet in-situ synthesis
3. Labelling of customer chromatin immunoprecipitated DNA
4. Hybridisation, washing and scanning of OGT ChIP microarrays
5. Data visualisation using the OGT ChIP browser
6. Data analysis and interpretation

Image of an OGT Escherichia coli ChIP array

Advantages of the OGT ChIP service

• We offer a comprehensive consultancy ChIP service from experimental design through all stages to data analysis and interpretation
• A flexible approach ensures that OGT will design and optimise probes to your specification. Probe designs may be promoter specific, a specific region of interest or a targeted region for probe selection.
• Fabrication using ink jet in-situ synthesis (IJISS) provides
• high quality microarrays at a cost effective price
• flexible synthesis technology which allows you to evaluate each array design and make changes as required
• Long 60mer oligonucleotide microarrays results in higher sensitivity, generating information rich data
• A high probe density giving you the ability to screen whole Prokaryotic genomes on one array at high resolution
• No sample PCR amplification reduces preparation time
• Two colour microarrays enable test and control samples to be hybridised on the same array saving valuable time and experimental costs
• Gives you the choice to run the microarray experiments in your laboratory or ours
• We can supply as few as one custom microarray or as many as hundreds in a cost-effective manner
• We have experience in data interpretation from ChIP experiments enabling you to understand the significance of your data
• We offer training in the microarray techniques required for ChIP experiments at our laboratory in Oxford.

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