Methylation profiling

OGT offer design, fabrication and processing of customised microarrays to determine the position of DNA methylation sites along the genome and to establish their effects on regulation in gene expression.

There are a number of different methods used to analyse methylated DNA using microarrays including:

• Methylation specific PCR¹. This combines methylation with sensitive restriction enzymes and PCR to distinguish methylated DNA from unmethylated DNA
• Treat DNA with bisulphate. This converts non-methylated cytosine to uracil leaving methylated cytosine unaltered. The cytosine to uracil conversion can be detected using a DNA microarray in a similar way to detecting SNPs.

OGT microarrays can be used to detect DNA produced using methylation specific PCR or following bisulphate treatment.

Reference:
¹Herman J.G., Graff J.R., Mycohanen S., Nelkin, B and Baylin S.B. (1996) Proc Natl Acad Sci USA 93 p9821-9826

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