Protein binding arrays
OGT has set up and optimised a protocol to convert synthesised single stranded oligonucleotides into double DNA duplexes to establish binding profiles of human transcription factors. During incubation of the purified DNA binding protein with the microarray the protein binds to the double stranded DNA. The protein can then be detected using Fluor labelled antibodies to the protein as in figure 1.
This technique differs from ChIP in that
- The binding of the DNA binding protein occurs in vitro and requires isolated protein
- The method is suitable for defining the sequence of a consensus binding site of a protein of interest.
OGT will design and fabricate a customised protein binding array specific to your DNA binding protein of interest. Ink-jet in-situ synthesis ensures the rapid and cost-effect production of high quality arrays. OGT's long oligonucleotide probes, 60mers, are suited to this application as the length of the oligonucleotides should be long enough to contain the protein's DNA binding sites.
Click here to read about the protein binding array project with Dr Ioannis Ragoussis, Head of Genomics, The Wellcome Trust Centre for Human Genetics, Oxford
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