Specials
OGT's microarray fabrication uses flexible chemistry to enable synthesis of:
- 3 prime to 5 prime
- 5 prime to 3 prime
- flexible oligonucleotide length
- linkers
5 prime to 3 prime oligonucleotides
The oligonucleotides on OGT customised microarrays are normally synthesised 3 prime to 5 prime, where the 3 prime end sits closest to the glass surface. However, the Ink jet in-situ synthesis (IJISS) technology employed by OGT also enables oligonucleotides to be synthesised using reverse phosphoramidites so that the oligonucleotides are built from 5 prime to 3 prime with the 5 prime end located closest to the glass surface. This makes the 3 prime end available for ligation or primer extension which is important in some applications.
Flexible oligonucleotide length
IJISS is a flexible platform offering:
- oligonucleotides of any length up to 60 nucleotides (60 mers)
- oligonucleotides of mixed length synthesised on the same microarray
- standard oligonucleotides (60 mers)
Contact OGT to discuss your requirements
Linkers
Linkers are short molecules that have been used to extend the oligonucleotide probe away from the surface of the microarray slide to improve hybridisation1.
If you require linkers to be incorporated in your microarray probes, contact OGT and we shall be happy to discuss your requirements.
Reference
1Shchepinov MS, Case-Green SC and Southern EM (1997) Nucleic Acids Res 25 p1155-1161
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