Specification Sheet: Arrays for eukaryotic gene expression
Specifications for Oxford Gene Technology (OGT) microarray service for eukaryotic gene expression studies
| Measurable | OGT eukaryote arrays |
| 360° QC | RNA QC upon sample arrival QC of labelling efficiency and incorporation LIMS sample tracking |
| Microarray platform | Agilent HD OGT design printed on Agilent SurePrint™ G3 microarrays |
| Species covered | All species covered by Agilent SurePrint arrays; includes custom eArray designs |
| Array density | 1x244k, 2x105k, 4x44k, 8x15k G3: 1x1M, 2x400k, 4x180k, 8x60k |
| Experimental design consultancy | Available on request |
| Sample preparation consultancy | Available on request |
| Starting sample | Total RNA, poly A+ RNA |
| Starting sample requirement | Two colour: Total RNA: 50 ng – 5 μg; poly A+: >10ng One colour: Total RNA: 200 ng – 1 μg; poly A+ >10ng |
| Sample QC method | Agilent 2100 Bioanalyzer: RNA LabChip NanoDrop™ 8000 spectrophotometer |
| Labelling method | Direct labelling – linear amplification (cRNA) |
| Slide format | 1x244k, 2x105k, 4x44k, 8x15k G3: 1x1M, 2x400k, 4x180k, 8x60k Printed using Agilent SurePrint technology |
| No. of controls | Number depends on array density, please enquire |
| Replicate no. of probes | Number depends on array, please enquire |
| Genome coverage | Whole genome with human, mouse, rat (4x44k); Bespoke coverage with eArray |
| Database version | Genome build: hg18 |
| Custom sequences | Available on request |
| Custom design by OGT | Available |
| Single/dual colour | Both |
| Detection limit | Two colour: 50 ng One colour: 200 ng |
| Sensitivity | Two colour: 1 in 7 million transcripts (based on molar ratio) One colour: 1 in 1.4 million transcripts (based on molar ratio) |
| Dynamic range | 5 logs |
| Data analysis | Tailored to customer requirements (e.g. GeneSpring® GX Software) |
| Data format | Tailored to customer requirements TIFF images, XML files, TXT files (raw data), customer report, relevant data analysis files (e.g. GeneSpring), QC report |
LIMS: laboratory information management system; QC: quality control; ULS: Universal Linkage System.
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