Specification Sheet: Arrays for prokaryotic gene expression

Specifications for Oxford Gene Technology (OGT) microarray service for prokaryotic gene expression studies

Measurable	OGT prokaryote microarrays
360° QC	RNA QC on sample arrival QC of labelling efficiency and incorporation LIMS sample tracking
Microarray platform	Agilent HD OGT design printed on Agilent SurePrint™ G3 microarrays
Species covered	All species covered by Agilent SurePrint arrays; includes custom eArray designs
Array density	1x244k, 2x105k, 4x44k, 8x15k G3: 1x1M, 2x400k, 4x180k, 8x60k
Experimental design consultancy	Available on request
Sample preparation consultancy	OGT provides advice on extraction of RNA from sample source
Starting sample	Total RNA
Starting sample requirement	Two colour: Total RNA 100 ng – 1 μg
Sample QC method	Agilent 2100 Bioanalyzer NanoDrop™ 8000 spectrophotometer
Labelling method	Indirect labelling – linear amplification (amino allyl UTP)
Slide format	1x244k, 2x105k, 4x44k, 8x15k G3: 1x1M, 2x400k, 4x180k, 8x60k Printed using Agilent SurePrint technology
No. of controls	Number depends on array density; available on request
Genome coverage	Depends on array designed, please enquire
Custom design by OGT	Available on request
Single/dual colour	Both available
Detection limit	100 ng
Dynamic range	5 logs
Data analysis	Available, depending on customer need. GeneSpring® GX Software can be used to investigate differentially expressed genes
Data format	Tailored to customer requirements TIFF images, XML files, TXT files (raw data), customer report, relevant data analysis files (e.g. GeneSpring), QC report

LIMS: laboratory information management system; QC: quality control.

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