Single molecule imaging
What difference does a single molecule make? It is either “very little” or “quite a lot”, depending on whether it is between 100 and 101 (only 1%), between 1 and 2 (50% or 100% change), or between zero and one.
Current tools such as microarrays provide insight into vast numbers of probes but do not have the sensitivity to detect single molecules. Current single molecule microscopes work well for detecting single molecules even in living cells but are not suited to study the large surface areas offered by microarrays. Most genomic and gene expression “single molecule” detection methods rely on some form of biological amplification which can introduce bias in the results.
We have developed and are using in our research a single molecule scanner with outstanding sensitivity providing the ideal platform to study the small number of molecules stemming from individual cells without resorting to biological amplification.
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