CytoSure™ Molecular Arrays

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Reliable detection of copy number changes for research into a range of genetic disorders.

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Overview

CytoSure Molecular Arrays are designed to accurately identify small intragenic copy number variations (CNVs) in genes associated with a variety of disorders. The content for each array has been designed and optimised in collaboration with leading molecular genetics experts at Emory University. In addition to pre-designed catalogue arrays, you can also utilise our extensive array design expertise to produce custom arrays matching your precise specifications.

Hear Dr Madhuri Hegde, Executive Director Emory Genetics Laboratory, discuss the development, optimisation and use and of CytoSure Molecular Arrays in our exclusive webinar.

CytoSure Molecular Arrays deliver:

  • Accurate detection of copy number variation — a perfect complement to sequencing analysis 
  • Array content fully optimised and research-validated by Emory University
  • Flexible array content and formats — create the ideal array for your needs
  • Cost-effective processing — multiple molecular disorders can be included in a single array
  • Easy data interpretation using optimised protocols and industry-leading CytoSure Interpret Software

Ordering Information

ProductContentsCat. No. Price
CytoSure Custom Molecular Array (various formats)
Microarray with a choice of formats; CytoSure Interpret Software. Content: Your choice of content

020018

Get a quote
CytoSure Molecular Array – Autism Spectrum Disorders (4x180k)
Microarray with four arrays of 180,000 spots; CytoSure Interpret Software.
Enquire
Get a quote
CytoSure Molecular Array – Cardiovascular Disorders (4x180k)
Microarray with four arrays of 180,000 spots; CytoSure Interpret Software.
Enquire
Get a quote
CytoSure Molecular Array – Ciliopathies (4x180k)
Microarray with four arrays of 180,000 spots; CytoSure Interpret Software.
Enquire
Get a quote
CytoSure Molecular Array – Congenital Abnormalities & Noonan Syndrome (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Congenital Disorders of Glycosylation (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Cystic Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Diabetes (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Duchenne Muscular Dystrophy (DMD) (8x60k) Microarray with eight arrays of 60,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Epilepsy (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Haemoglobinopathy (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Hearing Loss (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Inherited Cancers (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire  Get a quote
CytoSure Molecular Array – Inherited Eye Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Inherited Metabolic Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Intellectual Disability (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Leukodystrophy (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Lysosomal Storage Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software Enquire  Get a quote
CytoSure Molecular Array – Mitochondrial Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Neuro-Behcet Syndrome (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Neuromuscular Disorders (8x60k) Microarray with eight arrays of 60,000 spots; CytoSure Interpret Software.  Enquire Get a quote
CytoSure Molecular Array – Short Stature (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – X-Linked Intellectual Disability (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote

CytoSure™ products are for research use only; not for use in diagnostic procedures

Details

Accurate detection of copy number variation — a perfect complement to NGS analysis

Copy number variation (CNV) is a major cause of genetic disease and its analysis forms a vital part of the clinical research workflow. While Sanger sequencing and targeted next generation sequencing (NGS) is increasingly being used to detect specific point mutations, it is not possible to use these techniques to accurately detect CNV. As such, many molecular genetics researchers are choosing to complement sequencing studies with array comparative genomic hybridisation (aCGH) — the gold-standard for CNV detection.

Hear Dr Madhuri Hegde, Executive Director Emory Genetics Laboratory, discuss the development, optimisation and use and of CytoSure Molecular Arrays in our exclusive webinar: 

https://vimeo.com/78335114

CytoSure Molecular Arrays enable accurate CNV detection and analysis for research into a wide range of disorders (Table 1).

Disorders covered
Autism Spectrum Disorders Inherited Cancer
Cardiovascular Disorders
Inherited Eye Disorders
Ciliopathies Inherited Metabolic Disorders
Congenital Abnormalities & Noonan Syndrome
Intellectual Disability
Congenital Disorders of Glycosylation Leukodystrophy
Cystic Disorders
Lysosomal Storage Disorders
Diabetes
Mitochondrial Disorders
Duchenne Muscular Dystrophy (DMD)
Neuro-Behcet Syndrome
Epilepsy
Neuromuscular Disorders
Haemoglobinopathy
Short Stature
Hearing Loss
X-Linked Intellectual Disability

Table 1: Genetic disorders covered by CytoSure Molecular Arrays. 

Array content fully optimised and research-validated by Emory University

CytoSure Molecular Arrays have been designed and optimised in collaboration with leading molecular genetics experts at Emory University. Each gene is targeted by multiple exon-specific probes, allowing accurate detection of copy number variations (CNVs) encompassing single and multiple exons.

The probes for each gene have been selected from OGT’s proprietary Oligome™ database — a database of over 23 million in-silico optimised oligonucleotide probes. CytoSure arrays utilise 60mer oligonucleotide probes, which have been shown to offer higher signal-to-noise ratios though increased specificity and sensitivity1. To further improve performance, each array has been verified to contain no gaps in the gene coverage.

The optimised array design coupled with the powerful CytoSure Interpret Software that accompanies each array ensures sensitive detection of aberrations as small as a few kilobases within each gene exon (Figure 1).

Molecularfig1 000

 

 

 

 

 

 

 

Figure 1: CytoSure Interpret Software easily detects and displays small aberrations. Shown here is a 3.7kb deletion on chromosome 1 in exon 5 of the DBT gene, which, combined with a nonsense mutation, is causative of Maple Syrup Urine disease. Data kindly provided by Madhuri Hegde, Ph.D., FACMG, Emory University.

OGT has the experience and expertise, required to optimise the design of microarrays, which is why we chose to partner with them". Dr Madhuri Hegde, Scientific Director, Emory Genetics Lab, United States of America

Flexible array content and formats

CytoSure Molecular Arrays cover a wide range of molecular disorders (Table 1); however, the content and format of each array can be customised to suit your specific needs. Probes for multiple disorders can be combined in a single array, plus entirely new array content for disorders currently not covered can be included. All new probe content is optimised in-silico to ensure optimal performance.

Custom designed CytoSure arrays can utilise a variety of formats depending on your desired level of focus, with 1, 2, 4, or 8 arrays available per slide to provide the most cost-effective solution for your research (Figure 2). Processing can be undertaken in your lab or in OGT’s state-of-the art high-throughput laboratory in Oxford, UK.

array w760

 

 

 

 

 

 

 

Figure 2: Multiple arrays on a single slide can reduce costs and improve consistency. Depending on your desired level of focus you can design 1, 2, 4, or 8 arrays per slide for maximum flexibility.

Easy data interpretation

All CytoSure arrays are provided with CytoSure Interpret Software, a powerful, easy-to-use package for the analysis of aCGH data. Innovative features such as the Accelerate Workflow enable standardised and automated data analysis. In addition, extensive annotation tracks covering syndromes, genes, exons, CNV and recombination hotspots — each of which link to publically available databases — can be used to provide results in context.

The complete solution

All CytoSure arrays have been validated using CytoSure Genomic DNA Labelling Kits; these labelling kits have been uniquely developed and optimised to enable rapid delivery of high-quality results with excellent signal-to-noise ratios. Two formats are available; the CytoSure Genomic DNA Labelling Kit is sufficient for 24 samples and is ideal for labs running one or two arrays a week. For high-throughput labs, the CytoSure HT Genomic DNA Labelling Kit is recommended as its plate-based protocol allows simultaneous labelling of 96 samples. To achieve the best quality data possible, is it is recommended that CytoSure arrays are used in conjunction with CytoSure Genomic DNA Labelling Kits.

Ordering Information

ProductContentsCat. No. Price
CytoSure Custom Molecular Array (various formats)
Microarray with a choice of formats; CytoSure Interpret Software. Content: Your choice of content

020018

Get a quote
CytoSure Molecular Array – Autism Spectrum Disorders (4x180k)
Microarray with four arrays of 180,000 spots; CytoSure Interpret Software.
Enquire
Get a quote
CytoSure Molecular Array – Cardiovascular Disorders (4x180k)
Microarray with four arrays of 180,000 spots; CytoSure Interpret Software.
Enquire
Get a quote
CytoSure Molecular Array – Ciliopathies (4x180k)
Microarray with four arrays of 180,000 spots; CytoSure Interpret Software.
Enquire
Get a quote
CytoSure Molecular Array – Congenital Abnormalities & Noonan Syndrome (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Congenital Disorders of Glycosylation (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire  Get a quote
CytoSure Molecular Array – Cystic Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Diabetes (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Duchenne Muscular Dystrophy (DMD) (8x60k) Microarray with eight arrays of 60,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Epilepsy (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Haemoglobinopathy (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire  Get a quote
CytoSure Molecular Array – Hearing Loss (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Inherited Cancers (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire  Get a quote
CytoSure Molecular Array – Inherited Eye Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Inherited Metabolic Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Intellectual Disability (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Leukodystrophy (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Lysosomal Storage Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software Enquire  Get a quote
CytoSure Molecular Array – Mitochondrial Disorders (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Neuro-Behcet Syndrome (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote
CytoSure Molecular Array – Neuromuscular Disorders (8x60k) Microarray with eight arrays of 60,000 spots; CytoSure Interpret Software.  Enquire Get a quote
CytoSure Molecular Array – Short Stature (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire  Get a quote
CytoSure Molecular Array – X-Linked Intellectual Disability (4x180k) Microarray with four arrays of 180,000 spots; CytoSure Interpret Software. Enquire Get a quote

 

Related products

CytoSure Genomic DNA Labelling Kit 24 reactions: clean-up columns, dyes, nucleotide mix, random primers, enzyme, collection tubes 020022 Get a quote
CytoSure HT Genomic DNA Labelling Kit 96 reactions: 2 purification plates, nucleotide mix, random primers, enzyme, collection tubes 500040 Get a quote

CytoSure™ products are for research use only; not for use in diagnostic procedures

References

1. Curtis, C. et al (2009) The pitfalls of platform comparison: DNA copy number array technologies assessed. BMC Genomics 2009, 10:588