CytoSure™ ISCA Sample Tracking Spike-ins

Increasing numbers of aCGH samples combined with higher-throughput array formats means that it is imperative to track samples throughout the labelling, hybridisation and analysis process to maintain sample identity. CytoSure ISCA Sample Tracking Spike-ins are uniquely designed to enable reliable sample tracking and easy identification of sample mix-up using OGT’s class-leading CytoSure ISCA Arrays and CytoSure Interpret Software.

CytoSure ISCA Sample Tracking Spike-ins deliver:

• Confidence in results
• Simple one-step procedure with no alteration to existing workflows
• Easy identification of sample mix-up

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CytoSure™ products are for research use only; not for use in diagnostic procedures.

Confidence in results

As aCGH is now recognised as the first-tier test for research into numerous cytogenetic aberrations many laboratories are scaling up their processes to increase throughput and reduce costs. Parallel processing of higher numbers of samples increases the possibility of sample mix-up. Even automated workflows contain several steps where sample identity can be lost (e.g. pipetting samples into gasket slides). CytoSure ISCA Sample Tracking Spike-ins enable researchers to quickly and easily identify any erroneous samples ensuring only accurate data is reported.

Simple one-step procedure with no alteration to existing workflows

Each CytoSure ISCA Sample Tracking Spike-in is designed to a specific, unique region of the genome. Oligonucleotide probes complementary to the sample tracking spike-ins are included on all of the ISCA-approved arrays supplied and optimised by Oxford Gene Technology (OGT).  Eight different CytoSure ISCA Sample Tracking Spike-ins are available. Each spike-in has been carefully prepared to ensure that there is no cross-hybridisation with other probes on the array or with any other region on the genome. Each spike-in supplied is sufficient for 12 labelling reactions and is provided in 3 separate aliquots to avoid potential DNA degradation caused by repeated freeze-thawing. In addition, colour-coded caps are used for ease of identification, aiding correct usage. The spike-in is simply pipetted into the labelling reaction, then the labelling reaction and array processing continues following the standard workflow.  These spike-ins are particularly suitable for use when the labelling reactions are prepared in tubes and ideally complement the 24 reaction CytoSure Genomic DNA Labelling Kit.

Easy identification of sample mix-up

The specific sample tracking spike-in used is simply selected in the CytoSure Interpret Software and the system automatically confirms if the resultant data is correct for this spike-in, thereby identifying whether there has been a sample mix-up (Figure 1).

Figure 1: Fast and intuitive entry of sample tracking probe into CytoSure Interpret Software. Drop-down menus allow the user to quickly enter the specific sample tracking probe used in the labelling reaction. The identity of the sample tracking probe is clearly displayed.

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CytoSure™ products are for research use only; not for use in diagnostic procedures.