CytoSure™ ISCA Arrays

Every Cytosure ISCA array provides:

• Confident detection of syndrome-related aberrations through ISCA-endorsed high-density probe coverage of disease and syndrome relevant regions
• Reliable detection of other aberrations through high-resolution whole genome coverage
• A ready-to-run solution with a range of formats to suit your resolution, multiplexing and budget requirements
• Accurate results with high signal-to-noise ratios using proven 60-mer oligonucleotide
• Easy identification of sample mix-up

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CytoSure™ products are for research use only; not for use in diagnostic procedures.

Innovative array design

CytoSure ISCA Arrays comprise a research-validated collection of specific probes that enable reliable detection of copy number changes for a variety of molecular disorders. These arrays were designed in collaboration with the International Standards for Cytogenomic Arrays (ISCA) Consortium, to offer standardised, evidence-based formats to the cytogenetic research community.

Cost-effective processing

Each syndrome-related aberration is targeted by multiple specific probes, allowing accurate detection of copy number variations (CNVs). Through combining informative probes for multiple molecular disorders on a single array (Table 1), CytoSure ISCA Arrays allow immediate and accurate processing of diverse samples, thereby reducing both time to analysis and cost. 

Table 1: A selection of genetic disorders detected by CytoSure ISCA Arrays. For a full list click here.

The probes for these arrays have been selected from OGT’s proprietary Oligome™ database — a database of over 23 million in silico optimised oligonucleotide probes. CytoSure arrays utilise 60 mer oligonucleotide probes, which have been shown to offer higher signal-to-noise ratios through increased specificity and sensitivity.

Flexible array formats

CytoSure ISCA Arrays are available in three array formats 4 x 44k, 8 x 60k and 4 x 180k to match your resolution and throughput requirements. All CytoSure arrays have been validated using CytoSure Genomic DNA Labelling Kits; these labelling kits have been uniquely developed and optimised to enable rapid delivery of high-quality results with excellent signal-to-noise ratios. Two formats are available; the CytoSure Genomic DNA Labelling Kit is sufficient for 24 samples and is ideal for labs running one or two arrays a week. For high-throughput labs, the CytoSure HT Genomic DNA Labelling Kit is recommended as its plate-based protocol allows simultaneous labelling of 96 samples. To achieve the best quality data possible is it is recommended that CytoSure arrays are used in conjunction with CytoSure Genomic DNA Labelling Kits.

Effortless data interpretation 

All CytoSure arrays are provided with our complimentary, industry-leading CytoSure Interpret Software — a powerful, easy-to-use package for the analysis of aCGH data. Innovative features such as the Accelerate Workflow enable standardised and automated data analysis. In addition, extensive annotation tracks covering syndromes, genes, exons, CNV and recombination hotspots — each of which link to publicly available databases — provide results in context.

Easy identification of sample mix-up

As aCGH is now recognised as the first-tier test for research into numerous cytogenetic aberrations many laboratories are scaling up their processes to increase throughput and reduce costs. Parallel processing of higher numbers of samples increases the possibility of sample mix-up. Even automated workflows contain several steps where sample identity can be lost (e.g. pipetting samples into gasket slides). CytoSure ISCA, ISCA UPD and ISCA +SNP Arrays contain sample tracking probes which, when used in combination with CytoSure ISCA Sample Tracking Spike-ins, enable researchers to quickly and easily identify any erroneous samples, ensuring only accurate clinical data is reported.

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Related products

CytoSure™ products are for research use only; not for use in diagnostic procedures.