CytoSure™ Chromosome X Arrays

CytoSure Chromosome X Arrays were developed in close collaboration with leading cytogeneticists to deliver high-resolution detection of abnormalities on the X chromosome.

CytoSure Chromosome X Arrays deliver:

• A choice of formats and content
• High-resolution coverage of every gene on the X chromosome
• Easy data interpretation 

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CytoSure™ products are for research use only; not for use in diagnostic procedures.

High-resolution coverage of every gene on the X chromosome

In cytogenetics research, array CGH (aCGH) is increasingly being used for the identification of chromosomal aberrations associated with a number of related genetic disorders at a higher resolution than conventional non-array methods.

CytoSure Chromosome X Arrays provide comprehensive coverage of all the predicted genes, open reading frames, predicted miRNAs and rRNA genes on the X chromosome. This unique design enables enhanced detection of chromosomal abormalities due to denser coverage of syndrome relevant regions using optimal length 60mer oligonucleotides. The arrays also include control probes for chromosome 16 and 21.

Figure 1: The CytoSure Chromosome X (4x44k) Array in combination with CytoSure Interpret Software allowed the identification of a 1.5Mb deletion, which includes the Steroid Sulfatase (STS) gene.

A choice of formats and content

CytoSure Chromosome X Arrays are available in 4 x 44k and 2 x 105k formats. The 4 x 44k array offers an exon-centric design with 1 probe every 4kb. The higher-resolution 2 x 105k array offers a gene-centric design covering both exons, introns and flanking regions with 1 probe every 1.6kb.

CytoSure arrays utilise 60 mer oligonucleotide probes, which have been shown to offer higher signal-to-noise ratios though increased specificity and sensitivity¹. All CytoSure arrays have been research-validated using CytoSure Genomic DNA Labelling Kits; these labelling kits have been uniquely developed and optimised to enable rapid delivery of high-quality results with excellent signal-to-noise ratios. Two formats are available; the CytoSure Genomic DNA Labelling Kit is sufficient for 24 samples and is ideal for labs running one or two arrays a week.  For high-throughput labs, the CytoSure HT Genomic DNA Labelling Kit is recommended as its plate based protocol allows simultaneous labelling of 96 samples. To achieve the best quality data possible is it is recommended that CytoSure arrays are used in conjunction with CytoSure Genomic DNA Labelling Kits.

Easy data interpretation 

All CytoSure arrays are provided with CytoSure Interpret Software which is a powerful, easy-to-use package for the analysis of aCGH data. Innovative features such as the Accelerate Workflow enable standardised and automated data analysis. In addition, extensive annotation tracks covering syndromes, genes, exons, CNV and recombination hotspots — each of which link to publically available databases — can be used to provide results in context.

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Related products

CytoSure™ products are for research use only; not for use in diagnostic procedures.

References

1. Curtis, C. et al (2009) The pitfalls of platform comparison: DNA copy number array technologies assessed. BMC Genomics 10, 588-610