Specification Sheet: Genefficiency microRNA

Tuesday 23 August 2011

Specifications for Oxford Gene Technology (OGT) microarray service for miRNA studies

Measurable	Agilent v1 miRNA	Agilent v2 miRNA
360° QC	QC of RNA on arrival LIMS sample tracking
Microarray platform	Agilent SurePrint HD
Species covered	Mouse Human Rat
Array density	8x15k
Experimental design consultancy	Yes
Sample preparation consultancy	Yes
Starting sample	Total RNA
Starting sample requirement	100 ng: 260/280 >1.8 260/230 >1.8 25 ng/μl	100 ng: 260/280 >1.8 260/230 >1.8 50 ng/μl
Sample QC method	Agilent 2100 Bioanalyzer: NanoDrop 8000 spectrophotometer
Labelling method	Direct end labelling with Cyanine 3 pCp
Slide format	8x15k slide format printed using Agilent SurePrint technology incorporating hairpin probe design
Replicate no. of probes	16–20 (up to 4 different unique probe sequences per miRNA)
Genome coverage	Varies with species and array version
Database version	miRBase v10.1–v13
Design	Coupled probe design and labelling for optimal sensitivity and specificity
Custom sequences	No
Custom design	No
Single/dual colour	Single
Dynamic range	5 logs
Data analysis	Tailored to customer requirements e.g. statistical analyses, clustering, principal component analysis
Data format	Tailored to customer requirements, e.g. raw text result files through to full report with presentation of results, plus a QC report