Single cell analysis

Imagine....easy single cell results, 1000's of data points

Gene expression and miRNA analysis today has fundamental limiting steps

• the amount of starting material
• scanner resolution

Current solutions offer a crude estimate of gene expression by

• blending thousands or millions of cells and delivering an "averaged" gene expression result
• needing to amplify single cell amounts of mRNA/miRNA

No current method allows easy one-step preparation and analysis of single cell gene expression levels.

Current methods for single cell analysis include

• for preparation - FACs sorting, dilution
• for analysis - qRT-PCR/RT-PCR, FISH

These methods are

• multistep
• laborious in time
• difficult to repeat, and
• high in cost

making single cell analysis close to unachievable.